Anyone any idea on DNA detection????

PCR method.......

yes...
what do u nee to kno?

Got to design a complete process flow of DNA detection chip (lab-on-chip).
Starting from PCR to nano-wells to fluorescent tagging.

At this point, how to desing a lab-on-chip, inject solution into wells and detection of targeted DNA (micro-array, etc.)

oh i love PCR......with those small microfuge tubes,

http://www.gene-chips.com/

Thank You

1. Spectrophotometry:
The cheapest & easiest & simplest & quickest method is simply to measure OD (optical density) at 260nm and 280nm .
The ration of OD 260nm/280nm for pure DNA should be 1.8-2.0.

Spectrum of you sample might also give clue about the sample. DNA has a very charasteristic Absorption specturm.

2. Electrophoresis:
This is also another method where you run your DNA in Agarose Gel (0.75%-2% depending upon the size of your DNA, Bigger the DNA size less Agarose % you need) under the influence of Electric field (50-120V).

3.Physical Properties of DNA:
a. Melting Behaviour: If you always have the same DNA to detect, the melting behaviour of DNA might be also clue to detect DNA.
b. Viscousity: If you have genomic DNA and highly concentrated, & you are going to measure at constant condition. Then viscocity of the sample might help.

4. Protein DNA interaction: There are some proteins (enzymes) which are know to bind with DNA.

4. PCR:
This can be done only if you know the sequence of your DNA. For this you need to design primers, Enzyme polymerase, Neucleotides as reagents Besides you need also need thermocycler. This is possible only in research institute.

4.DNA sequencing:
This is also possible if you know the source or the sequence of your DNA. Possilble only in research institute.

I think for you purpose you simply need to measure OD at 260nm & 280nm and the ratio of them will tell you about rest.

Gosh i totally forgot about electrophoresis for DNA using agarose and ethidium bromide ..i use polyacralymide gel for protein detection...
i don't have that much idea on microarrary. My freind used to do microarray for her research but i don't have that much idea..sorry..

Thank you for helping me out.

I came through different methods of DNA detection during my search, and if you like to i don't have any problem sharing with you.

Babajee can you share over here which method you came through ???
I have experience in some of the above methods..though i don't do my own research i do for my professor's research,,,are you a PHD student?

I came with following sequential process flow: injection of target ssDNA followed by PCR (i did not wanted to do that cuz you can get away with it, but have to as my prof. wants to), microfluidics, micro-array, and fluorescent response.
This is what I have come up with and I am pretty sure it work. I need to talk to my group though.
No, I am not a PhD student.